The RFM3200 measures the temperature of the chip on the tag. The chip has a small thermal mass, and it generally heats or cools to the ambient temperature within seconds.
In terms of the time required to read the tag, this can depend on a lot of things, like the RFID reader used, the reader settings, the number of tags in the area, the distance between the reader and tags, and other factors. Generally speaking though, you can get a single temperature reading from a tag around 2-10 times per second.
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Axzon (formerly RFMicron)
3700 N. Capital of Texas Hwy
Austin, TX 78746
Tel: (512) 535-4647 Axzon.com